Research
Below are a few research papers on EM Technology® that we have made available on our website. Much more information is available through paid peer-review sites, EMRO Japan, and various published books available through EM America and elsewhere.
EM Research Organization maintains an online database of over 300 research papers and articles on EM Technology® from around the world. All the information is in English and varies from published to non-published materials and it is available to the public free of charge. Use simple keywords or phrases to find the articles all in Acrobat PDF format.
Good dental offices make extensive diagnosis of each tooth as to pocket depth, tissue color and swelling. The greater the pocket depth, the looser the teeth, the poorer the color leaning toward stagnation, loss of texture and the greater the inflammation or swelling of the gums. The clinical tests are from different offices with no economic benefits other than to observe the results to see if the products are beneficial to their patients. The dental professionals instructed the patients to use Dr. Don's® Antioxidant toothpowder and mouthwash instead of their regular toothpaste and mouthwash. One office used the mouthwash as the disinfectant in water-pik, an essential spray rinse to reach deep into the periodontal tissues. We wanted to know the bad results as well as the good to determine what may be the cause of the bad results. Since, the technology and science is radically different from conventional dentistry, there were only a few offices that chose to participate in the clinical tests. There are still on going tests and we will document them on this website. One office is doing extensive tests that may last several months.
Testing Analysis
Dentists use an average of mean pocket depth in analysis to compare the results. They normally take the typical three readings per side of the tooth or a total of 6 readings per tooth. Since clinically, they would see some aspects of periodontal condition would be more involved say in the palatal side, we wanted to see if they got an even response or a isolated response since patients have a tendency to brush mainly on the buccal front surface of the teeth and ignore or brush poorly on the palatal lingual back surface of the teeth.
Looking at the mean sulcus pocket depth, they get an overall view of improvement or non-significant progress. Since in the clinical setting, they would see in a healthy mouth, no more than 3 mm pocketing and usually only 2mm pocketing on the side of the teeth, for a pocket average of +2.5mm. The higher from that number, the more disease we would see. They then took the amount of potential improvement to ideal and compare the actual amount to determine total state of health of the gums. The pocket average is an indication of total condition of the gums.
Case #101. Woman Age ___, with history of 2 previous Non-surgical scaling and root planing treatments in the past.
Patient presented with advanced boneloss, moderate subgingival calculus and moderate bleeding upon probing. Pockets presented as 1 to 9 mm. Gingiva at margins was generally red and mildly inflammed. Patient brushed and flossed regularly.
After 4 quadrants of non-surgical scaling and root planing therapy, patient was sent home with a bottle each, of Dr. Don's® Oral Rinse and Toothpowder. The patient was instructed to follow the directions on the labels and to also put a capful of the Gargle in her waterpik water to be used one time daily.
At a one month follow-up periodontal maintenance prophy, the patient presented a generalized 1 to 4 mm reduction in pocket depths and only 10 slight bleeding points. Gingival tissue appeared firmer, more stippled, and tighter. Color was a healthy pink. Inspite of the deep red of the Oral Rinse, there appeared to be no staining of the teeth. Infact, they appeared brighter than patients who had been using chlorhexidine or stannous rinses for the same time frame.
An additional benefit seemed to be that the patient reported she had far less sensitivity than she had ever had in her previous scaling and root planing experiences. She said this very much surprised her as she had been dreading facing the pain again.
Patient took home another bottle of the Oral Rinse and another Toothpowder and placed on a 3 month recare. It will be very exciting to follow her progress.
Case 102 Male 50 years old
The patient has been a patient of record for eight years. He came in with initial periodontal condition which we were able to resolve with shorten recall appointments. He then was unable to return due to financial challenges as prescribed. When returning he had an advanced class III perio-condition with a Class II mobility on tooth number 18. We referred the patient to a periodontist for evaluation. Prognosis was hopeless for tooth no 18, poor for the posterior teeth 2,3,14,15, 19 and 32 and fair for teeth 4-13 and 30 and 31.
Recommended treatment of extraction of 18 and scaling and root planning to be followed by re-evaluation with surgery a strong possibility. He chose to participate in this trial picking up the products and returned for evaluation one month later. No other treatment was done except his general cleaning on 5/5 and perio-evaluation on 6/24. On 7/23, he was evaluated by our office. The change was significant with little or no bleeding upon probing. The probing depth on 6/24 had a mean pocket depth of 4.45 mm on the buccal of the maxillary. On 7/26, it was 3.1mm on the buccal. On the palatal and it started out as 4.95 mm and in one month it was 3.2 mm. The buccal on the mandible started out at 4.7mm when re-evaluated it was 2.77 mm and the lingual started ate 4.8 mm and improved to 2.77 mm. The mean total for the entire mouth started ate 4.725 mm average of pocket depth and within one month with no other intervention, it improved to 3.03 mm depth. A 1.69 mm improvement with no noticeable bleeding. A 75% impressive improvement.
Case 103 Male 54 years old
The individual is a patient that originally came in 7 years ago and had undergone scaling and root planing at that time. Although a shorten recall had kept his condition under control , through time he felt he only needing cleanings twice a year. His condition of a perio Class III had returned upon his visit of 6/4. We read the pockets at that visit and he chose to engage in this trial. We gave him the product with instructions and he returned 7/15 for re-evaluation of his situation.
The maxillary average buccal pocket depth was 3.47mm on 7/15, it was 2.83 mm. The palatal was 3.41 mm on 7/15 it was 3.06 mm. The mandible buccal was 3.54 mm starting but on 7/15 ut was 3,00 nn, /tge kubgayak started at ab average if 3,729 mm and on 7/15, it was 3,20 mm. For an average pocket depth of 3.53 mm throughout the mouth and on 7/15 an average of 3.02 mm with no bleeding and no other treatments. A 25-50% improvement throughout.
Case 104 Female Age 79 years old
This case is a perio Class IIIa-IV. She has a history of full mouth perio surgery years before with a guarded prognosis. We have been able to maintain her teeth through frequent cleanings. Lazer therapy was done several times in October and November with limited success. She spends winters in the South, so treatment options have only been during a 6-7 month period of time. She seeks no other care during he time spent away. She was evaluated on 6/9 and given the products with instructions. She was the only patient to use a sonicare toothbrush with the application.
Her starting pocket depth on the maxillary buccal pocket was 3.066 mm, it improved to 2.066 mm. Her palatal started at 3.566 mm and improved to 3.1 mm. Class IIIs pocket readings on the mandible (where she has bone disease) showed the buccal average pocket depth of 2.37 before and after. The lingual started at 2.66 mm and improved to 2.48 mm. The most impressive result was the total elimination of bleeding.
Conclusions
Dr. Don's® Antioxidant toothpowder and mouthwash appear to be clinically significant for the improvement of gum disease. Since there a variety of cases, we would have expected some cases not to respond due to the complication of health issues. They all responded favorable and consistently within a short period of time. The greatest improvement from this clinical analysis was the reduction of bleeding and inflammation, but this does not lend to a numerical analysis.
EM-X® application as radio-protector, i.e. radio-protective mean, much work was carried out here, when the primary positive results were obtained, because the dosage and the scheme of EM-X® application was not known. The radio-protective effect will be confirmed in this presentation with the establishment of two EM-X® properties: antioxidant and immunocorrecting.
It is known that the organism exposure leads to the activation of peroxidation system with formation of hyperoxides and decrease of antioxidant system function. In given case, the acute gamma-irradiation of animals was accompanied by the accumulation in blood of malonic dialdehide, one of principal products of lipid peroxidation. Simultaneously, the decrease of blood antioxidant activity was observed.
The EM-X® application after the irradiation (2.5 ml/100 g of weight) prevented the malonic dialdehide accumulation in blood and decrease of antioxidant activity of blood. The two fold increase of EM-X® dosage did not strengthen the effect. This indicates the importance of selecting the optimum EM-X® dose, as with EM•1®. Even better results were obtained at chronic irradiation. The activity in blood erythrocytes of suproxide dismutase, one of the principal enzymes of antioxidant protection, decreases after the chronic irradiation, like the antioxidant activity of blood.
Use of EM-X® during the irradiation normalized the level of superoxide dismutase activity. The effect of EM-X® use in 9 ml/100 g dosage was more significant than in 13 ml/100 g dosage. The activity of catalase, the second enzyme of organism antioxidant system, did not decrease during the first month after irradiation. However it became higher at EM-X® introduction than it was before the irradiation. This can be interpreted as protective reaction of organism. The same results were obtained in the conditions of animals confined within the 30-km zone, where they underwent chronic irradiation. The irradiation of the organism, the rise of hydroperoxides was observed as well as the lowering of the antioxidant system in the organism. It is important to stress here that there were no considerable differences in the normalization of indices in EM-X® doses 45.0 and 125 ml/100 g in a month, Besides, EM•1® application in 45 ml/100 g dose in a month gave the same results as EM-X® application. The analogous normalizing effect of EM•1® and EM-X® at the stay of animals in 30-km zone was also discovered regarding the change of catalase activity in blood erythrocytes and antioxidant activity in blood. Another important item was that in the experiments on chronic irradiation of animals and the confinement of animals in 30-km zone, the decrease of mass of the spleen was found (one of principal organs in immunogenesis).
The application of EM-X® protected the mass of spleen from decrease at the irradiation. Taking this into account, attempts to protect the immune system on the conditions of irradiation was undertaken this year. The receptor, activation and proliferative properties of blood lymphocytes were studied. The confinement of animals in the zone conditioned the rise of proliferative ability of lymphocytes. Use of EM-X® in a bigger dose and EM•1® as well decreased this effect stimulated by the irradiation. The state of function of T-and B-cellular receptors decreases at chronic irradiation. Application of EM-X® in a bigger dose as well as of EM•1® caused protective effect. Similar data were obtained regarding the functional state of receptors to interleukin-2, when we could decrease the injuring action of radiation using EM-X® and EM•1®. One more conclusion from the data of studied indices that EM•1® does not yield to EM-X® by the efficiency in the effect on immune system state. Thereby EM-X® and EM•1® have the radio-protective effect, activating the system of antioxidant protection and possessing immunocorrecting action.
From: Prospects of Utilizing Effective Microorganisms (EM•1® and EM-X®) in the Liquidation of Nuclear Accident Consequences E.F. Konoplya Institute of Radiobiology of National Academic of Sciences of Belarus . Found in: Clinical and Basic Research on EM-X®, Volume 4.
EM-X® ceramics come in various shapes and sizes. All the ceramics, except for the Terra C powder are EM-X® ceramics. The ringstones are normally used in drinking water to help structure or energize the water. All the ceramics have varying effects on the ORP in the water. Essentially, they all have an antioxidation effect on the water.
From Eco Pure Digest 2004 (English):
"EM-X® Ceramics change the electric characters of water into states good for our health.
This is indicated by a graph of redox (oxidation-reduction) potential. It shows if water is in an oxidized state or not. A larger positive value indicates that the water is more oxidized. Human body fluids and blood have redox values of about 250~ -250mV. I is said that a person is healthier if the values are negative. The graph above indicates that the value of tap water is about 700 mV, while it goes dow to around 200 mV afte the water passes through Reductive Ceramics. This shows that the Reductive Ceramic (Kangen) changes unhealthy, highly oxidized water into healthy water. "
Abstract: Dematolon is an electronic instrument which measures the electronic potential of living body of a membrane in symmetry regions such as arms and legs.When the electronic potential meter indicates 50, the potential is in a normal condition. When it's higher than 50, it indicates an inflammation condition, and below 50 indicates an abnormal condition.
Patients who use mobile phones and computers are exposed to the electromagnetic waves, and which lead the potential to indicate as low as 40 to 10 in many kinds of internal organs.
When EM-X® Ceramics [are] applied within the radius of 1m in diameter from the patient, the electromagnetic potential again indicated a good condition of the immunological defense functional value even it is affected by the irradiation of electromagnetic waves.
Amilcar Salgado Lopez, Columbia.
EM Medical Conference, 2002, Okinawa, Japan. Pp. 51-53.
A Case for EM-X® Rice Bran Beverage as an Antioxidant Prophylactic Agent
The general wellness of individuals and management of human clinical conditions in which a role for free radicals and oxidants are implicated continues to underpin the search for non-toxic antioxidants. Free radical reaction of lipid peroxidation is an important issue in the food industry where manufacturers minimize oxidation in lipid containing foods by use of antioxidants during the manufacturing process-foods are produced that maintain their nutritional quality over a defined shelf life.
There is recruitment of immune and inflammatory cells, which are activated to produce mediators of inflammation including oxidants and cytokines, such as the pro-inflammatory cytokine tumor necorsis factor-alpha (TNF-alpha). Oxidative stress has been implicated in the pathogenesis of several inflammatory lung disorders. One consequence of this process is to enhance the expression of both pro-inflammatory and protective antioxidant genes. Oxidants and inflammatory mediators such as tumour necrosis factor-alpha (TNF-alpha) activate transcription factors such as NF-kB and Activator Protein-1 (AP-1). Interleukin-8 (IL-8) is a ubiquitous inflammatory chemokine that mediates a multitude of inflammatory events in the lung. Hydrogen peroxide (H2O2) (100um) and TNF-alpha (10ng/ml) imposed oxidative stress in A549 cells as shown by depletion of the antioxidant reduced glutathione (GSH) concomitant with increased levels of oxidised glutathione (GSSG). EM-X® Rice Bran Beverage inhibited bot hH2O2 and TNF-alpha-mediated activation of NF-kB and AP-1. Both H2O2 and TNF-alpha significantly increased IL-8 release, which was inhibited by pre-treatment of A549 cells with EM-X® Rice Bran Beverage compared to the control untreated cells. This study shows that EM-X® Rice Bran Beverage inhibits both the oxidant H2O2 and the pro-inflammatory mediator, TNF-alpha induced IL-8 and suggests a mechanisms for the anti-inflammatory effects of EM-X® Rice Bran Beverage. We conclude that EM-X® Rice Bran Beverage may have theraputic potential in Neuroinflammation and inflammatory lung diseases.
Okezie I. Aruoma and Irfan Rahman. Presented at the first International EM Medical Conference, 2001.
Effects of EM-X® Rice Bran Beverage on the metabolisms of free radicals and the nervous conduction velocity in type II diabetes were studied. The serum SOD in the type II diabetes patients was significantly lower and the serum MDA was significantly higher that those in the normal control. After conventional plus EM-X® Rice Bran Beverage treatment the serum SOD in the patients was significantly increased by 21% and serum MDA decreased by 22%, indicating an antioxidant activity in the diabetic patients. Upon conventional plus EM-X® Rice Bran Beverage treatment the sensory conduction velocity was significantly increased by 8.3% in the median nerve and by 10% in the fibular nerve. The motor conduction velocity showed also an increasing tendency although no significance was observed. It might be due to the smaller sample and the shorter term of application of EM-X® Rice Bran Beverage.
Presented by: Bin, Ke, Su Xian, Zuojie Luo, Yinfen Qin, Hanlei Shen, Higa, Teruo. Page 34, Clinical and Basic Medical Research on EM-X®- A Collection of Research Papers (Volume I).
We examined the anti-HIV activity of Effective Microorganisms&trade(EM-X® [EM-X® Rice Bran Beverage]) in vitro. EM-X® is a new biological response modifier (BRM) that was originated through a combination of aerobic and anaerobic microorganisms. EM-X® Rice Bran Beverage inhibited HIV-1 replication in mononuclear cells (MNC).
MNCs were isolated from peripheral blood of healthy donors and infected with HIV-1 (HTLVIIIB, 3000 pg p24 equivalent/106 cells). Infected cells were incubated in the presence of EM-X® Rice Bran Beverage (0-100ml/ml) for 7d at 37ºC and culture medium was collected to assess for viral particles produced by MNC using ELISA for HIV-1 anitgen. The results showed that EM-X® Rice Bran Beverage inhibited HIV-1 reproduction in a dose dependent manner in all subjects. EM-X® Rice Bran Beverage at low concentration of 12.5ul/ml and 25ul/ml resulted in 24% and 40% ihibition respectively. Fifty percent inhibiation of viral replication was achieved at concentrations of 50-100ul/ml. Cytotoxicity of EM-X® Rice Bran Beverage was evaluated at MTT assay and results showed EM-X® Rice Bran Beverage is no toxic to MNC at the concentrations used (1-100m/ml). We concluded that EM-X® Rice Bran Beverage is a potent BRM with possible theraputic value as an anti-HiV agent without cytotoxic side effects.
Presented by: Mamdooh Ghoneum, Kim Choong, and Teruo Higa at the 1st International EM Medical Conference, Okinawa Japan, November 2001.
The entire paper is available in the Clinical and Basic Medical Research on EM-X®- A Collection of Research Papers (Volume 2). Pp. 27-39.
Nobuyuki Sato* and Teruo Higa***College of Global Environmental Sciences, Interdisciplinary Innovation, IOND University, Hawaii, USA
**College of Agriculture, University of the Ryukyus, Okinawa, Japan.
Introduction
Infections today are caused by mechanisms different from those of past infections. And, that time until disease onset also tends to increase. These diseases definitely show a new trend different from the diseases seen in the past. One of these causes is considered to be environmental pollution together with the generation of various types of free radicals such as the activated oxygen species.
To effectively eliminate these free radicals that have adverse effects on the body, a group of compounds called antioxidants are attracting a great deal of attention recently. These substances include three types: substances that directly remove free radicals in scavenge manner, substances that promote the activities of free radical eliminating enzymes called SOD, and lastly SOD-like compounds. As for the antioxidation effect of EM-X®, recent studies have confirmed clearly that is directly remove(s) bad types of free radicals. In this presentation, the results of research on the components of EM-X® and the nature of its mechanisms of action, including those conducted in the past five years, will be presented. Whe hope to communicate these data to clinicians so that they may use this product with confidence.
Research on Antioxidant Capability and Effects of EM-X® Rice Bran Beverage
Fig. 1 and 2 show the research methods for the studies conducted in collaboration with the Radiation Chemistry Research Center, Shizuoka University and Nuclear Reactor Research Center, Kyoto University. The first experiment using not ver y strong Y rays and irradiation was conducted for a relatively long duration, and then we studied the effects of irradiation on the double helix structure of super-coil type DNA derived from E. coli. In this experiment, electrophoresis was conducted first. From the Separated fingerprints, we input the data with an image scanner to find out the proportion of CC type DNA that maintains a double helix structure and OC type DNA that shows a broken down structure. From these areas, the proportion of residual CC type DNA was calculated. The results showed that over 90% of CC type DNA remained intact in the presence of EM-X® Rice Bran Beverage, showing very potent antioxidation effect of EM-X® Rice Bran Beverage.
ESR measurement and data analysis: The sample was taken out of liquid nitrogen and rapidly returned to room temperature. Then the sample was inserted in the cavity and measurement was started. The time immediately after sample was melted was taken as time O, and measurement was started 20 seconds later. Seven measurements were made at intervals of 21.5 seconds. The ESR spectrum was measured at each time point. The time-related changes were plotted and extrapolated to find the ESP spectral intensity at 1 second immediately after the sample was melted. This value became smaller when EM® samples had greater anti-oxidation capability. The intensity of DMPO-OH after the addition of EM® material was determined compared to the DMPO-OH value before addition of EM®.
In the other study, free radicals were generated with very strong irradiation, with simultaneous binding of DNOP and OH. We studied how much OH was eliminated by EM-X® Rice Bran Beverage during this process. Table 1 shows the data of residual CC type DNA as an indication of the rate of radiation protection. From the data of CC type and OC type DNA obtained in this study, it is clear that a large proportion of CC type DNA, which is the genetic filament that has not received radiation damage, remain intact.
